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Susceptibility of full-length β-globin mRNA and decay intermediates to cap hydrolysis and cap analog competition for binding to eIF4E and cap antibody. Cytoplasmic RNA from Thal10 cells was treated without (−) (lanes 4 and 6) or with (+) (lanes 5 and 7) TAP (A) or Dcp2 (B). RNA recovered from each reaction was then incubated without (lanes 4 and 5) or with (lanes 6 and 7) a 5′-monophosphate-dependent 5′ exonuclease and analyzed by S1 nuclease protection assay.
