TABLE 1.
Cell cycle distribution of cells expressing phosphorylation site mutant CK2α proteins 12 h after release from a thymidine block
| Cell line and treatment | % of cellsc
|
||||
|---|---|---|---|---|---|
| Sub-G1 | G1 | S | G2a | Mb | |
| CK2α-HA | |||||
| Plus tetracycline | 0.64 | 32.04 | 13.77 | 48.79 | 4.01 |
| Minus tetracycline | 0.59 | 29.89 | 15.16 | 49.60 | 4.13 |
| CK2α-4D-HA | |||||
| Plus tetracycline | 0.36 | 28.91 | 17.97 | 46.79 | 4.85 |
| Minus tetracycline | 7.92 | 33.21 | 24.98 | 32.27 | 0.97 |
| CK2α-4E-HA | |||||
| Plus tetracycline | 0.44 | 31.71 | 15.47 | 44.08 | 7.26 |
| Minus tetracycline | 7.85 | 33.05 | 22.77 | 34.40 | 1.23 |
| CK2α-4A-HA | |||||
| Plus tetracycline | 0.41 | 31.22 | 16.36 | 44.67 | 6.80 |
| Minus tetracycline | 0.35 | 31.29 | 15.45 | 46.59 | 5.91 |
a
Cells with 4N DNA content negative for phospho-histone H3 staining.
b
Cells with 4N DNA content positive for phospho-histone H3 staining.
c
Boldface indicates significant differences between samples with and without tetracycline (P < 0.01).