TABLE 2.
Cell cycle distribution of nocodazole-arrested cells expressing phosphorylation site mutant CK2α proteins
| Cell line and treatment | % of cellsa
|
|||
|---|---|---|---|---|
| Sub-G1 | G1 | S | G2/M | |
| CK2α-HA | ||||
| Plus tetracycline | 7.00 | 6.33 | 10.57 | 88.26 |
| Minus tetracycline | 7.01 | 6.04 | 11.50 | 73.50 |
| CK2α-4D-HA | ||||
| Plus tetracycline | 3.89 | 3.13 | 10.88 | 71.80 |
| Minus tetracycline | 4.28 | 7.33 | 17.00 | 63.29 |
| CK2α-4E-HA | ||||
| Plus tetracycline | 3.40 | 5.81 | 11.41 | 76.86 |
| Minus tetracycline | 4.92 | 8.91 | 18.84 | 73.59 |
| CK2α-4A-HA | ||||
| Plus tetracycline | 5.24 | 5.57 | 12.37 | 82.64 |
| Minus tetracycline | 14.87 | 10.93 | 24.74 | 51.41 |
a
Boldface indicates significant differences between samples with and without tetracycline (P < 0.01).