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. 2009 Feb 17;29(8):2011–2022. doi: 10.1128/MCB.01472-08

FIG. 3.

FIG. 3.

Ang2/Tie2 signaling activates the PI3K/Akt pathway. (A) PI3K activities of HUVECs treated with Ang2 siRNAs. Forty-eight hours after HUVECs in 10-cm petri dishes were transfected with NC, Ang2-82, or Ang2-84 siRNA, cell lysates were prepared for analysis of PI3K activity as described in Materials and Methods (means ± standard deviations; n = 3). (B and C) Total and phospho-Akt levels of HUVECs treated with Ang2 siRNAs. Phospho-Akt and total Akt were compared in NC and Ang2-84 siRNA-treated HUVECs. Western blots of three independent samples are depicted, along with the P/T ratio of Akt (n = 3). (D) Exogenous Ang2 induces Akt phosphorylation. HUVECs were stimulated with Ang2 at the indicated concentrations for 30 min, and phospho- and total Akt levels were compared. Ang1 (100 ng/ml) was used as a positive control. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control. *, P value of <0.05; **, P value of <0.01.