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. 2009 Feb 2;29(8):2264–2277. doi: 10.1128/MCB.01484-08

FIG. 1.

FIG. 1.

GFRα1 expression after the adenovirus-mediated transfer of Ape1/Ref-1 in GM00637 cells. (A) The GM00637 cells were transfected with Ad-LacZ or Ad-Ape1/Ref-1 at a multiplicity of infection of 50, and the cells were harvested 48 h after the infection. The total RNA was extracted and subjected to semiquantitative RT-PCR using the Ape1/Ref-1-, GFRα-, and GAPDH-specific primers. (B) Protein extracts prepared 48 h after the infection with Ad-LacZ or Ad-Ape1/Ref-1. A 20-μg portion of the total protein was loaded onto an SDS-polyacrylamide gel for Western blot analysis. Antibodies to Ape1/Ref-1 and GFRα1 were used. The detection of α-tubulin was used as the loading control. (C) The GM00637 cells were transfected with control pcDNA3 vector (control), repair Ape1/Ref-1 mutant expression vector (ΔRD), or redox Ape1/Ref-1 mutant expression vector (ΔAPD), and the total RNA was then extracted and subjected to semiquantitative RT-PCR using the Redox Ape1/Ref-1, Repair Ape1/Ref-1, GFRα, and GAPDH primers.