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. 2009 Feb 2;29(8):2264–2277. doi: 10.1128/MCB.01484-08

FIG. 5.

FIG. 5.

Knockdown of Ape1/Ref-1 decreased pancreatic tumor cell invasion and pro-MMP-9 expression in response to GDNF. (A) Photomicrographs showing the effect of Ape1/Ref-1 siRNA and GFRα1 siRNA on the GDNF-induced increase in BXPC3 and MIA PaCa-2 invasion. The histograms show the average number of invading cells. (B) Photomicrographs showing the influence of the GFRα1 lentivirus on the GDNF-induced invasion of Ape1/Ref-1 siRNA-transfected cells. The histograms show the ability of the GFRα1 lentivirus to rescue the GDNF-induced invasion of cells expressing Ape1/Ref-1 siRNA. The experiments for panels A and B were repeated three times, each in duplicate. The data shown are means ± the SD. **, P < 0.01. (C) MIA PACa-2 cells were transfected with mock, control, Ape1/Ref-1, or GFRα1 siRNA. At 48 h after transfection, cells were incubated with GDNF (30 ng/ml) for an additional 24 h. Whole-cell lysates were prepared and subjected to immunoblotting with anti-Ape1/Ref-1, anti-GFRα1, anti-pro MMP9, or anti-β-actin antibodies.