Abstract
During a 15-month period, two methods for detection of herpes simplex virus (HSV) in 699 clinical specimens were compared: (i) 24-well-plate centrifugation (24WPC) with MRC-5 cells and staining with type-specific monoclonal antibodies (Syva Co., Palo Alto, Calif.) after incubation for 16 to 18 h and (ii) conventional tube cell culture with primary rabbit kidney and A549 cells. HSV was identified by conventional tube cell culture in 165 (24%) of 699 specimens and by the 24WPC method in 116 (17%) of 699 specimens. One specimen was positive for HSV by the 24WPC method alone, compared with 50 specimens positive only by conventional cell culture (P less than 0.0001). The sensitivity, specificity, and positive and negative predictive values of the 24WPC technique with MRC-5 cells for detection of HSV in clinical specimens were 70, 99.8, 99, and 91%, respectively. Centrifugal inoculation of MRC-5 cells in 24-well plates and staining with monoclonal antibodies after incubation for 16 to 18 h is an insensitive means of detecting HSV in clinical specimens and should not replace conventional tube cell culture with primary rabbit kidney cells.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Callihan D. R., Menegus M. A. Rapid detection of herpes simplex virus in clinical specimens with human embryonic lung fibroblast and primary rabbit kidney cell cultures. J Clin Microbiol. 1984 Apr;19(4):563–565. doi: 10.1128/jcm.19.4.563-565.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gleaves C. A., Wilson D. J., Wold A. D., Smith T. F. Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation. J Clin Microbiol. 1985 Jan;21(1):29–32. doi: 10.1128/jcm.21.1.29-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moore D. F. Comparison of human fibroblast cells and primary rabbit kidney cells for isolation of herpes simplex virus. J Clin Microbiol. 1984 Apr;19(4):548–549. doi: 10.1128/jcm.19.4.548-549.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pruneda R. C., Almanza I. Centrifugation-shell vial technique for rapid detection of herpes simplex virus cytopathic effect in Vero cells. J Clin Microbiol. 1987 Feb;25(2):423–424. doi: 10.1128/jcm.25.2.423-424.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Salmon V. C., Turner R. B., Speranza M. J., Overall J. C., Jr Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining. J Clin Microbiol. 1986 Apr;23(4):683–686. doi: 10.1128/jcm.23.4.683-686.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thiele G. M., Bicak M. S., Young A., Kinsey J., White R. J., Purtilo D. T. Rapid detection of cytomegalovirus by tissue culture, centrifugation, and immunofluorescence with a monoclonal antibody to an early nuclear antigen. J Virol Methods. 1987 Jul;16(4):327–338. doi: 10.1016/0166-0934(87)90018-8. [DOI] [PubMed] [Google Scholar]
- Winter G. F., Inglis J. M., Cubie H. A. Rapid diagnosis of herpes simplex virus infections in conventional and shell vial cell cultures using monoclonal antibodies. J Virol Methods. 1987 Mar;15(4):329–330. doi: 10.1016/0166-0934(87)90156-x. [DOI] [PubMed] [Google Scholar]
- Zhao L. S., Landry M. L., Balkovic E. S., Hsiung G. D. Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining. J Clin Microbiol. 1987 Aug;25(8):1401–1405. doi: 10.1128/jcm.25.8.1401-1405.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]