Fig. 3.
Effect of phenylephrine on glutamatergic eEPSCs of lamina II neurons elicited from primary afferents. A, original traces of eEPSCs show identification of monosynaptic (left) and polysynaptic (right) eEPSCs in two separate lamina II neurons evoked by electrical stimulation of the dorsal root entry zone (20 Hz). B, original traces showing that 50 μM phenylephrine decreased the amplitude of polysynaptic eEPSCs of one neuron in a reproducible manner. Note that conduction failure was indicated by the arrow. C, Summary data show the reproducible effect of phenylephrine on the amplitude of both monosynaptic and polysynaptic eEPSCs of lamina II neurons. Data are presented as means ± S.E.M. *, P < 0.05 compared with the baseline control. Phl, phenylephrine.