Table 1.
Proteins identified as differentially expressed (>2-fold change) in metastatic M4A4 and nonmetastatic NM2C5 cell lines using a 2-D liquid-phase method and MS and/or MS/MS
| Identity | Accession number | Database MW/pI | Experimental |
|||
|---|---|---|---|---|---|---|
| M4A4 Mr | NM2C5 Mr | MALDI % Cov. | Fold change | |||
| Up-regulated in M4A4 cells | ||||||
| Pyruvate kinase, isozymes M1/M2 | P14618 | 57937/8.0 | 57939 | 57925 | 26 | 34.6 |
| 60S Ribosomal protein L24 (ribosomal protein L30) | P83731 | 17779/11.3 | 17783 | 17781 | 33 | 4.9 |
| 40S Ribosomal protein S16 | P62249 M | 16445/10.2 | 16315 | 16316 | 47 | 4.2 |
| 28 kDa Heat- and acid-stable phosphoprotein | Q13442 | 20630/8.8 | 20660 | 20664 | 35 | 4.0 |
| Hematological and neurological expressed gene 1-like protein variant a; ST1a | 19070523 Ma) | 16015/5.5 | 16007 | 16005 | 37 | 3.7 |
| Elongation factor Tu, truncated | P49411 | 49542/7.3 | 45095 | 45085 | 33 | 2.8 |
| NADH-ubiquinone oxidoreductase 13 kDa-B subunit | Q16718 | 13459/5.8 | 13372 | 13371 | 40 | 2.8 |
| T-complex protein 1, eta subunit | Q99832 | 59367/7.6 | 59436 | 59468 | 20 | 2.5 |
| Nuclease sensitive element-binding protein 1 | P67809 M | 35924/9.9 | 35839 | 35838 | 55 | 2.3 |
| Superoxide dismutase [Cu-Zn] | P00441 | 15936/5.7 | 15891 | 15894 | 61 | 2.1 |
| 60S Ribosomal protein L4 | P36578 | 47698/11.1 | 47658 | 47655 | 13 | 2.1 |
| 40S Ribosomal protein S9 | P46781 | 22592/10.7 | 22463 | 22461 | 21 | 2.1 |
| Up-regulated in NM2C5 cells | ||||||
| Annexin A2 | P07355 | 38604/7.6 | 38517 | 38519 | 37 | 14.6 |
| Annexin A1 | P04083 | 38715/6.6 | 38673 | 38686 | 39 | 14.5 |
| 60 kDa Heat-shock protein, truncated | P10809 | 61055/5.7 | 57966 | 57967 | 47 | 11.1 |
| Heat-shock cognate 71-kDa protein (HSC70/HSP73) | P11142 | 70899/5.4 | 70960 | 70964 | 35 | 4.8 |
| T-Complex protein 1, β subunit | P78371 | 57489/6.0 | 57486 | 57493 | 43 | 4.3 |
| Heat-shock 70 kDa protein 1 (HSP70-1) | P08107 | 70053/5.5 | 70068 | 70069 | 38 | 4.1 |
| ATP synthase alpha chain, truncated | P25705 | 59751/9.2 | 55196 | 55210 | 20 | 4.0 |
| Alpha-endosulfine | O43768 | 13389/6.6 | 13299 | 13300 | 52 | 3.8 |
| Tumor protein D54 (hD54) (D52-like 2) | O43399 | 22238/5.3 | 22360 | 22278 | 58 | 3.5 |
| ATP synthase β chain, truncated | P06576 | 56560/5.3 | 51827 | 51844 | 36 | 3.3 |
| Platelet-activating factor acetylhydrolase IB b subunit | P68402 M | 25569/5.6 | 25527 | 25524 | 31 | 2.8 |
| Myosin light polypeptide 6 | P60660 | 16930/4.6 | 16875 | 16874 | 51 | 2.7 |
| Ubiquitin-conjugating enzyme E2 variant 2 | Q15819 | 16363/7.8 | 16275 | 16276 | 45 | 2.7 |
| Putative RNA-binding protein 3 | P98179 | 17171/8.9 | 17213 | 17217 | 45 | 2.6 |
| Calcyclin-binding protein | Q9HB71 | 26210/8.3 | 26123 | 26126 | 29 | 2.4 |
a)
Accession number in NCBI database.