Table 2.
Proteins detected either in M4A4 or NM2C5 cell lines by MS and/or MS/MS
| Identity | Accession number | Database MW/pI | Experimental |
|||
|---|---|---|---|---|---|---|
| M4A4 Mr | NM2C5 Mr | MALDI% Cov. | INa) | |||
| Detected only in M4A4 cells | ||||||
| Galectin-1 | P09382 | 14716/5.3 | 14715 | nd | 58 | 29.1 |
| Triosephosphate isomerase | P60174 | 26670/6.4 | 26541 | nd | 56 | 29.1 |
| Nucleoside diphosphate kinase B | P22392 | 17298/8.5 | 17213 | nd | 48 | 24.0 |
| 54 kDa Nuclear RNA- and DNA-binding protein | Q15233 | 54101/9.0 | 54277 | nd | 38 | 12.9 |
| Phosphoglycerate mutase 1 | P18669 | 28804/6.7 | 28718 | nd | 38 | 10.7 |
| Phosphoglycerate kinase 1 | P00558 | 44615/8.3 | 44591 | nd | 36 | 8.0 |
| Peroxiredoxin 2 | P32119 | 21892/5.7 | 21811 | nd | 39 | 7.2 |
| 39S Ribosomal protein L13, mitochondrial (L13mt) | Q9BYD1 | 20692/9.2 | 20604 | nd | 81 | 5.3 |
| GST P | P09211 | 23356/5.4 | 23269 | nd | 34 | 2.4 |
| My016 protein | Q9H3K6 | 10117/6.1 | 10162 | nd | 61 | 1.8 |
| Tubulin β-5 chain (tubulin 5 β) | P04350 | 49631/4.8 | 49409 | nd | 21 | 0.9 |
| Dihydropteridine reductase | P09417 | 25804/6.9 | 25743 | nd | 22 | 0.2 |
| Detected only in NM2C5 cells | ||||||
| Stress-induced-phosphoprotein 1 (STI1) | P31948 | 62640/6.4 | nd | 62866 | 15 | 3.6 |
| Heat-shock 27 kDa protein | P04792 | 22783/6.0 | nd | 22864 | 63 | 2.6 |
| Mannose-6-phosphate receptor-binding protein 1 | O60664 | 47033/5.3 | nd | 46954 | 49 | 2.4 |
| Eukaryotic translation initiation factor 4H | Q15056 | 27254/6.9 | nd | 27336 | n/a | 0.5 |
a)
IN, normalized intensity. For quantitation of each protein, the peak area of proteins were normalized against the peak area of bovine insulin internal standard
nd = not detected
n/a = not applicable