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. 2000 Jan 4;97(1):168–173. doi: 10.1073/pnas.97.1.168

Figure 2.

Figure 2

PKA is required for FGF-2-induced expression of the syndecan-1 gene (SYN-1). (a) Inhibition of PKA by H-89 blocks the FGF-2-induced transcription of the syndecan-1 gene. Wild-type NIH 3T3 cells (NIH 3T3wt) deprived of serum were treated for 30 min with H-89 (10 μM) before 8-hr FGF-2 stimulus, followed by Northern analysis of mouse syndecan-1 mRNA. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was used as a loading control. (b) Inhibition of PKA activity by expression of PKARΙ-mut inhibits the FGF-2-induced transcription of the syndecan-1 gene. Production of PKARΙ-mut was induced by application of ZnSO4 (50 μM final concentration) into the culture medium 3 hr before 8-hr FGF-2 (10 ng/ml) stimulus. (c) In nontransfected cells, ZnSO4 had no effect on the FGF-2-induced syndecan-1 transcription.