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. 2000 Jan 4;97(1):168–173. doi: 10.1073/pnas.97.1.168

Figure 5.

Figure 5

FGF-2 induces intracellular relocalization of the PKA catalytic subunit to the nuclear membranes and into the nucleus. (a) Quantification of PKAC in nuclear proteins of FGF-2-stimulated NIH 3T3 cells by Western blot analysis. Serum-starved NIH 3T3 cells were treated with FGF-2 (10 ng/ml) or PKA activator 8-Br-cAMP (200 μM) for 1 hr, and nuclear proteins were isolated. Ten micrograms of nuclear proteins was separated in 10% SDS/PAGE, blotted onto a nitrocellulose filter, and detected with anti-PKAC antibody. (b) Detection of PKAC in FGF-2 stimulated NIH 3T3 cells by indirect immunofluorescence confocal microscopy. Serum-starved NIH 3T3 cells were treated with FGF-2 or PKA-activator 8-Br-cAMP (200 μM) for 1 hr, fixed, and stained with anti-PKAC.