Figure 5.

Vav-2 is a substrate of the PDGF receptor in fibroblasts. (A) NIH 3T3 fibroblasts either were left untreated or were treated with PDGF, and lysates were immunoprecipitated with anti-Vav-2 antibody as indicated. Washed immunoprecipitates were resolved by SDS/PAGE, transferred onto nitrocellulose, and Western blotted with an anti-phosphotyrosine antibody. Coimmunoprecipitated PDGFR and Vav-2 are indicated by arrows. (B) Anti-Vav-2 immunoprecipitates from a parallel experiment to show equal loading of Vav-2.