Table 1.

Summary of identification of proteins from the gel in Fig. 1

Protein band analyzed	Molecule identified	Identification by MALDI	Number of peptide matches	Sequence coverage, %	Identification by MS/MS
1	Shc	+	25	40	ND
2	p62	−	0	0	+
3	p85	+	26	32	+
4	Vav-2	+	18	26	+
	Hrs	−	12	14	+
5	Hrs	+	22	19	+
	Cbl	+	30	38	+
	Eps15	−	14	14	+
6	Novel protein	+	37	35	+
	EGFR	+	22	21	+
7	EGFR	+	43	36	ND

Gel slices from the silver-stained gel shown in Fig. 1 labeled 1 through 7 were excised, digested with trypsin, and subjected to MALDI mass spectrometry followed by nanoelectrospray MS/MS. This table lists the molecules identified from each gel slice. Some proteins were identified from more than one gel slice. Number of tryptic peptides observed by MALDI analysis that matched tryptic peptides derived from a given protein in the database is shown. Sequence coverage refers to the percent of a given protein that corresponded to the tryptic peptides detected by MALDI analysis. ND, not done.