Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Dec;31(12):3170–3173. doi: 10.1128/jcm.31.12.3170-3173.1993

Evaluation of Biolog for identification of members of the family Micrococcaceae.

J M Miller 1, J W Biddle 1, V K Quenzer 1, J C McLaughlin 1
PMCID: PMC266370  PMID: 8308109

Abstract

The Biolog Identification System (Biolog, Inc., Hayward, Calif.) was challenged at two separate laboratories with 113 coded isolates, including 33 type strains of staphylococci, 5 strains of Micrococcus spp., and 1 strain of Stomatococcus mucilaginosus. Test parameters between the sites were controlled as much as possible. Discrepancies were arbitrated by using conventional biochemicals. Overall accuracies (correct to the species level) upon initial testing were 47.7 and 59.3%, respectively, at the two laboratories. After repeat testing of isolates generating "no identification" responses or errors, the overall accuracies increased to 69.0 and 74.3% at the two sites, respectively, revealing no significant difference in the final results at the two laboratories (78 of 113 versus 84 of 113; P > 0.05). Error rates were 7.1% at one site and 9.7% at the other. The Biolog is not yet accurate enough to serve as a primary method for identifying staphylococci.

Full text

PDF
3170

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banerjee S. N., Emori T. G., Culver D. H., Gaynes R. P., Jarvis W. R., Horan T., Edwards J. R., Tolson J., Henderson T., Martone W. J. Secular trends in nosocomial primary bloodstream infections in the United States, 1980-1989. National Nosocomial Infections Surveillance System. Am J Med. 1991 Sep 16;91(3B):86S–89S. doi: 10.1016/0002-9343(91)90349-3. [DOI] [PubMed] [Google Scholar]
  2. Bannerman T. L., Kleeman K. T., Kloos W. E. Evaluation of the Vitek Systems Gram-Positive Identification card for species identification of coagulase-negative staphylococci. J Clin Microbiol. 1993 May;31(5):1322–1325. doi: 10.1128/jcm.31.5.1322-1325.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Garibaldi R. A., Cushing D., Lerer T. Risk factors for postoperative infection. Am J Med. 1991 Sep 16;91(3B):158S–163S. doi: 10.1016/0002-9343(91)90362-2. [DOI] [PubMed] [Google Scholar]
  4. Hamoudi A. C., Marcon M. J., Cannon H. J. Evaluation of rapid identification of gram-positive cocci in positive blood cultures by use of the AutoMicrobic system Gram-Positive Identification Card. J Clin Microbiol. 1984 Aug;20(2):171–174. doi: 10.1128/jcm.20.2.171-174.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hussain Z., Stoakes L., Stevens D. L., Schieven B. C., Lannigan R., Jones C. Comparison of the MicroScan system with the API Staph-Ident system for species identification of coagulase-negative staphylococci. J Clin Microbiol. 1986 Jan;23(1):126–128. doi: 10.1128/jcm.23.1.126-128.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kloos W. E., George C. G. Identification of Staphylococcus species and subspecies with the MicroScan Pos ID and Rapid Pos ID panel systems. J Clin Microbiol. 1991 Apr;29(4):738–744. doi: 10.1128/jcm.29.4.738-744.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lämmler C. Characterization of Staphylococcus hyicus with the ATB 32 Staph system and with conventional tests. J Clin Microbiol. 1991 Jun;29(6):1221–1224. doi: 10.1128/jcm.29.6.1221-1224.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miller J. M. Evaluating biochemical identification systems. J Clin Microbiol. 1991 Aug;29(8):1559–1561. doi: 10.1128/jcm.29.8.1559-1561.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller J. M., Rhoden D. L. Preliminary evaluation of Biolog, a carbon source utilization method for bacterial identification. J Clin Microbiol. 1991 Jun;29(6):1143–1147. doi: 10.1128/jcm.29.6.1143-1147.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rhoden D. L., Hancock G. A., Miller J. M. Numerical approach to reference identification of Staphylococcus, Stomatococcus, and Micrococcus spp. J Clin Microbiol. 1993 Mar;31(3):490–493. doi: 10.1128/jcm.31.3.490-493.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stager C. E., Davis J. R. Automated systems for identification of microorganisms. Clin Microbiol Rev. 1992 Jul;5(3):302–327. doi: 10.1128/cmr.5.3.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stoakes L., Schieven B. C., Ofori E., Ewan P., Lannigan R., Hussain Z. Evaluation of MicroScan Rapid Pos Combo panels for identification of staphylococci. J Clin Microbiol. 1992 Jan;30(1):93–95. doi: 10.1128/jcm.30.1.93-95.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES