Figure 4.

The AtVPS45-silenced plants secrete ctVSD-containing cargo. A, Lines expressing GFP-CHI and ALEU-GFP were crossed with wild-type and siVPS45-10d plants, and the adaxial side of leaves from F1 plants was analyzed by confocal microscopy. Bars = 10 μm. B, Samples of apoplastic fluids (Apo) collected from rosette leaves of wild-type and siVPS45-10d plants were analyzed with the indicated antibodies. Total proteins (Total) from leaves of the same plants were analyzed to determine the expression levels of the proteins. The precursor (p), intermediate (i), and mature (m) forms of CPY are marked. C, The apoplastic fluids and total proteins from rosette leaves of the indicated mutants were analyzed with anti-AtAleurain antibodies (ALEU). evti11, enhanced vti11; evti12, enhanced vti12 (Sanmartin et al., 2007). Positions of molecular mass markers (in kD) are shown at left in B and C. Wt, Wild type; si10d, siVPS45-10d.