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Western-blot detection of the α-catalytic subunit. A, Total protein extracts (T) and chloroplast-extracted proteins (C) were separated by SDS-PAGE and transferred to nitrocellulose membranes. Immunodetection of AKIN10 and AKIN11 was performed using the enhanced chemiluminescence kit. B, The membrane in A was reblotted using antibodies against Rubisco.
