Figure 7.

RabE cosuppression adversely affects Arabidopsis plant size and leaf morphology. A, Western blot (with anti-RabE antibody) illustrating how RabE-cosuppressed plants (lane 3) have a considerably lower amount of endogenous RabE protein compared with both the wild type (lane 1) and GFP-RabE1d overexpressors (lane 2). B, RabE-cosuppressed plants (top and bottom right) have smaller size and altered morphology compared with wild-type Arabidopsis (top left) and RabE1d-overexpressing plants (bottom left). C, Enlarged image of the RabE-cosuppressed plant in B (top right); arrowheads point to the wavy leaves. D, Size comparison of rosette leaves from 5-week-old RabE-cosuppressed (three left) and wild-type (two right) Arabidopsis plants. Bar = 10 mm. E, RT-PCR analysis of expression of the five RabE and four RabD genes in wild-type (lane a) and in RabE-cosuppressed (lane b) Arabidopsis plants. Equal volumes of the PCR samples were loaded on a 1% agarose gel. The gel was photographed with a Bio-Rad imager, and Quantity One software was used to quantify the bands. Intensity values, normalized to those of Actin8, are represented in the chart as percentages of the wild-type value.