Figure 2.

cam3 mutant exhibited significantly decreased thermotolerance. A, cam2, cam3, and cam4 seedlings showed no differences from wild-type seedlings under normal growth conditions. However, when 6-d-old seedlings were exposed to 45°C for 50 min and then recovered at 22°C for another 6 d, only cam3 exhibited decreased thermotolerance as compared with cam2, cam4, and wild-type plants. B, Survival rate of the wild-type, cam2, cam3, and cam4 mutant plants after HS at 45°C for 50 min. Each value is the mean ± se of three biological replicates, with 30 seedlings per experiment. C, AtCaM protein levels in wild-type, cam2, cam3, and cam4 mutant plants after HS at 37°C for 2 h. Tubulin was used as an internal loading control. Quantification of the bands is shown below. WT, Wild type.