Figure 3.

Time course of AtCaM3 expression during HS and spatiotemporal expression of the AtCaM3 gene in Arabidopsis. A, Ten-day-old AtCaM3∷GUS seedlings were heat stressed at 37°C for 0 to 4 h. GUS activity was determined as described in “Materials and Methods.” Data are means ± se from four different AtCaM3∷GUS transgenic lines with three biological replicates. B, GUS staining showing the expression pattern of AtCaM3 in a 10-d-old seedling (a), a rosette leaf (b), a cauline leaf (c), a silique (d), an anthotaxy (e), a flower (f), a stamen (g), a petal (h), and a sepal (i). Bars = 1 mm in a to c, e, and f, and 0.1 mm in d and g to i.