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. 2009 Apr;149(4):1773–1784. doi: 10.1104/pp.108.133744

Figure 4.

Figure 4.

Transformation of the cam3 mutant with 35S∷AtCaM3 rescued the decreased thermotolerance. A, RT-PCR analysis of ectogenous AtCaM3 transcripts in wild-type, cam3 mutant, and three cam3/AtCaM3 transgenic lines (1-2, 7-4, and 43-1). Actin was used as an internal control. B and C, Wild-type, cam3 mutant, and three cam3/AtCaM3 transgenic lines under normal (B) and HS (C) conditions. For the thermotolerance assay, 6-d-old seedlings grown at 22°C were shifted to 45°C for 50 min and then returned to 22°C for 6 d. D, Survival rate for wild-type, cam3 mutant, and three cam3/AtCaM3 transgenic lines (1-2, 7-4, and 43-1) after HS at 45°C for 50 min. Each value is the mean ± se of three biological replicates, with 30 seedlings per experiment. E, TBARS levels for wild-type, cam3 mutant, and three cam3/AtCaM3 transgenic lines (1-2, 7-4, and 43-1). Plants were heat stressed at 45°C for 60 min and then returned to 22°C for 2 d for recovery. TBARS levels were determined relative to the wild-type controls. Each value is the mean ± se of three biological replicates. WT, Wild type.