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. 2009 Apr;149(4):1773–1784. doi: 10.1104/pp.108.133744

Figure 5.

Figure 5.

Overexpression of AtCaM3 improves thermotolerance. C, Vector control; WT, wild type; 3-7, 3-21, and 3-22 are three AtCaM3-GUS-overexpressing transgenic lines transformed with 35S∷AtCaM3-GUS. A, GUS activity in wild type, vector control, and three transgenic lines. Each value is the mean ± se of three biological replicates. B and C, Wild-type, vector control, and three transgenic lines under normal (B) and HS (C) conditions. For the thermotolerance assay, 6-d-old seedlings grown at 22°C were shifted to 45°C for 70 min and then returned to 22°C for 6 d, at which time they were photographed. D, Survival rates for the wild-type, vector control, and three transgenic lines. Survival was determined 6 d after heat stress at 45°C for 70 min. Each value is the mean ± se of three biological replicates, 30 seedlings per experiment. E, TBARS levels of wild-type, vector control, and three transgenic lines. Plants were heat stressed at 45°C for 60 min and then allowed 2 d at 22°C for recovery. TBARS levels were normalized relative to the wild-type control. Each value is the mean ± se of three biological replicates.