Figure 7.

Effect of AtCaM3 on the accumulation of AtHSP18.2. Ten-day-old seedlings were heat stressed at 37°C for 2 h or maintained at 22°C as a control. Total protein was extracted, separated by SDS-PAGE, and analyzed by western blotting. Tubulin was used as an internal quantification control. A, Wild-type plants at 22°C; wild type plants and cam3 mutants after HS. B, Wild-type plants and three independent cam3/AtCaM3 transgenic seedlings (1-2, 7-4, and 43-1) exposed to 37°C. C, Wild-type plants and three AtCaM3-GUS-overexpressing transgenic seedlings (3-7, 3-21, and 3-22) exposed to 37°C. D, Quantification of the bands in A to C. C, Control; WT, wild type.