Auxin involvement in shade avoidance responses of petioles to low R:FR and hypocotyl responses to low blue light photon fluence rates. A to E, Petioles in control and low R:FR light. F to I, Hypocotyls in control and low blue light. A to C and F to H, Auxin activity, shown with the auxin-responsive pIAA19∷GUS reporter (A, B, F, and G) and IAA19 gene expression (C and H), is increased upon low R:FR and low blue exposure. D, E, and I, Undisturbed auxin signaling and transport are required for shade avoidance responses to low R:FR (D and E) and low blue light photon fluence rates (I). Data are means ± se (n = 8–12 for petioles, n = 30–50 for hypocotyls). Different letters indicate significant differences (P < 0.05). [See online article for color version of this figure.]