Table 2.

Comparison of QRT-PCR and QPCR assays

Dilution	Ct value	SD	Ct value change	SD
QPCR
10	23.87	0.02
102	27.16	0.17	3.29	0.17
103	30.47	0.31	3.30	0.48
104	33.93	0.20	3.46	0.11
QRT-PCR
10	20.60	0.01
102	23.77	0.08	3.17	0.08
103	26.82	0.14	3.06	0.18
104	29.53	0.12	2.70	0.12
105	32.99	0.76	3.47	0.80

Each citrus sample was divided into two Eppendorf tubes with 0.1 g each for DNA or RNA extraction and dissolved into 100 μl of water, respectively. 1 μl of DNA or RNA samples was used in either QPCR or QRT-PCR assays respectively. Each value is the mean of three replicates.