Figure 2.

Venn diagrams of proteins identified from 2D gels derived from three different membrane fractions (A) and proteins with more than one transmembrane domain (TMD) identified in 2D gel vs. 2D-LC-MS/MS experiments (B). Acronyms are described in the flowchart of Figure 1. 2D gel-based protein identifications included several Y. pestis growth conditions, while those identified from 2D-LC-MS/MS efforts were limited to stationary phase cell growth at 26°C. The analysis of TMDs is based on bioinformatic motif predictions by the algorithm TMHMM.