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. 2009 Apr 10;4(4):e5190. doi: 10.1371/journal.pone.0005190

Figure 1. Co-delivery of CT induces IL-17-producing CD4 T cells to bystander peptide antigen.

Figure 1

After transfer of OT-II splenocytes, the recipient B6 mice were immunized once with 40 µg of OVAp II together with CpG (30 µg) or CT (0.2 µg or 2 µg) intranasally. (A) Lung tissues were isolated at day 7 and the frequencies of donor OT-II cells were measured by CD4, Vα2 and Vβ5 staining. (B) The lung mononuclear cells were stimulated with OVAp II or PMA/ionomycin, or CTB peptide (D) and stained for CD4, IFN-γ and IL-17. Only CD4+ gated cells were shown in the dot plots. (C) The graphs indicate the average ratio of IL-17:IFN-γ expression of CD4+ gated cells. Dot plots are representative of at least three independent experiments and data are average ± SEM, n = 4∼5 mice per group.