Figure 4.

In vivo renoprotection by Xe versus renal IRI. Mice were pretreated with hydrodynamically injected siRNA (or scrambled siRNA or PBS) and 24 h later were exposed to 70% N₂ or 70% Xe balanced with O₂ for 2 h. IRI was by bilateral clamping of the renal pedicle for 25 min, 24 h after gas exposure. (A) Quantification of histologic scoring after IRI in mice pretreated and exposed to various gas mixtures treatments (n = 6). (B and C) Attenuation by HIF-1α siRNA pretreatment of the renoprotective effect of Xe in IRI as reflected by plasma creatinine (B) and plasma urea (C; n = 6). (D) Mean value of TUNEL-positive cells in renal sections after various treatments (n = 6). NegsiR, negative siRNA; HIFsiR, HIF-1α siRNA. Data are means ± SD. *P < 0.05, **P < 0.01 versus control.