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Sorting and expansion of Sca-1+ or Sca-1− KSCs. (A) Representative FACS plot showing Sca-1+ and Sca-1− cells in passage 2 before flow sorting. (B) Equal numbers of purified Sca-1+ and Sca-1− cells were cultured and counted daily. Cell expansion was calculated as the daily cell number divided by the initial cell number.
