Figure 8.

KSC-DC stimulated T cell proliferation. (A and B) LPS-stimulated C57BL/6 KSC-DCs or control DCs were isolated from transwell co-culture on day 5 and mixed with CFSE-labeled CD4⁺ BALB/c T cells (allogeneic response; A) or OT-II transgenic T cells plus OVA323-339 peptide (nominal antigen antigen-specific response; B). Proliferation is expressed as percentage mitosis, as determined by loss of CFSE fluorescence by flow cytometric analysis. Proliferation was significantly less with KSC-DC versus control DC stimulators in either culture system. **P < 0.01 versus control wells.