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. 2009 Apr 17;5(4):e1000393. doi: 10.1371/journal.ppat.1000393

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Lung samples from naïve, (B) S. mansoni i.v. egg challenged (primary) and (C) i.p. egg sensitized and challenged (secondary) Retnla^−/− mice were snap frozen and embedded in OCT. In situ Retnla expression was evaluated by LacZ reporter activity (blue precipitate) in frozen tissue sections exposed to β-galactosidase substrate, X-gal. Original magnification ×20. Small inset in panel C magnification ×10.