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Performance of feedback protocol. A, Median firing rate (dish-wide) achieved versus target. Any firing rate (FR) between the spontaneous rate and 500 SPSA could be stably maintained. Higher rates were not achievable in this culture without exceeding the safe voltage limit of 900 mV. The dotted line marks equality of achieved and target rates. Data are from five series on different sets of electrodes; 45 DIV. B, Stimulus voltage used to control firing rate at different levels.
