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Northern blot of xPRLR mRNA expression at different developmental stages. (A) Whole tadpole poly(A)-RNA; (B) Tail total RNA. The blots were hybridized first with the xPRLR probe and then the PR28 probe, which is used to standardize RNA loading (29). The marks on the left indicate the positions of the ribosomal RNAs. The numbers on top of the lanes are tadpole stages (18).
