Fig. 3.

Effects of cholesterol supplementation on transduction efficiency (TE) of NIH3T3 cells using either FBS (A) or FP (B), each at a serum concentration of 5% or 10%. 0.03 mM cholesterol was added at periods P + I + II (Fig. 1). A range of TE values was obtained by using different volumes of viral vector to transduce the target cells. All values were normalized to those under control condition at each collection, in which the same volume of viral vector produced without cholesterol supplementation was used to transduce the cells.