Fig. 5.

Effects of adding MbCD alone during vector production on transduction efficiency using vectors from the 2nd collection. MbCD with the same concentration (0.25 mM) as that used for cholesterol supplementation was added either alone (0.25 mM MbCD) or complexed with cholesterol (0.03 mM cholesterol) to 293-gag-pol cells during vector production. The insert figure represents the results when 5 mM unloaded MbCD was added to 293-gag-pol cells 9 h before the 2nd collection. All values were normalized to those under control condition at the 2nd collection, in which the same volume of viral vector produced without cholesterol supplementation was used to transduce the cells. Data points represent the mean ± S.D. (n = 2). Results are shown separately for cultures with low (20–40%) and high (>40%) transductions efficiency under control conditions.