Fig. 6.

Gag protein amounts in viral vectors determined by immunoblot for both control conditions and cholesterol supplementation. A typical immunoblot result for p30^gag in viral vectors from the 1st and 2nd collections with or without 0.03 mM cholesterol supplementation is shown in (A). For culture with cholesterol, cholesterol was added at periods P + I + II (Fig. 1). In (B) the amount of p30^gag (determined from densitometry analysis of immunoblots) in viral vectors with or without cholesterol supplementation for each collection was normalized to that of the 1st collection under control conditions. Data points represent the mean ± S.D. (n = 3). p = 0.26 for the 1st collection and p = 0.35 for the 2nd collection with or without cholesterol supplementation.