Fig. 8.

Phospholipid composition in untransfected 293-gag-pol cells with or without cholesterol supplementation. Packaging cell line 293-gag-pol (without transfection) was incubated with 0.03 mM cholesterol for 3, 9, 23, or 46 h, respectively. Alternatively, cholesterol-free methyl-beta-cyclodextrin (10 mM) was added to 293-gag-pol cells and incubated for 30 min at 37 °C to deplete cholesterol (depletion). LPC: lysophosphatidylcholine; SM: sphingomyelin; PC: phosphatidylcholine; PI: phosphatidylinositol; PS: phsphatidylserine; PE: phosphatidylethanolamine; PA: phosphatidic acid. Data points represent the mean ± S.D. (n = 2).