Table 2.

Statistical analysis of the benefits of cholesterol supplementation on transduction efficiency of NIH3T3 cells under different conditions (FBS or FP, the 1st or 2nd collection, 5% or 10% serum). Control condition in the table indicates that the same volume of viral vector produced without cholesterol supplementation was used to transduce the cells at each collection. The numbers in the table represent p values obtained using a two-tailed, unpaired t-test with equal variances.

Condition	Transduction efficiency under control conditions
	0–10%	10–20%	20–40%	>40%
FBS 10% 1st collection vs. control	<0.05	<0.05	<0.05	<0.05
FBS 10% 2nd collection vs. control	<0.05	<0.05	<0.05	0.07
FBS 10% 2nd collection vs. FBS 10% 1st collection	0.39	<0.05	<0.05	0.12
FBS 5% 2nd collection vs. FBS 5% 1st collection	n/a	<0.05	<0.05	n/a
FP 10% 1st collection vs. control	n/a	0.10	<0.05	0.65
FP 10% 2nd collection vs. control	<0.05	<0.05	0.39	0.80
FP 10% 2nd collection vs. FP 10% 1st collection	0.39	0.87	0.20	0.30
FP 5% 2nd collection vs. FP 5% 1st collection	n/a	0.07	0.80	n/a