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ULK1 phosphorylates Atg13 in vitro. (A and B) ULK1-FLAG (Wt) and kinase-dead ULK1K46N-FLAG mutant (KD) were immunoprecipitated from HEK293T cells cultured in the starvation medium for 1 h. The resulting precipitates were subjected to immunoblot analysis (A) or ULK1 kinase assay (B) using GST-Atg13 and GST (negative control) as substrates.
