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The AP-3 pathway is impaired in the Vps41 S-A mutant. Localization of GFP-Nyv1-Snc1TMD in Vps41 wild-type and mutant cells. Cells expressing GFP-tagged Nyv1-Snc1TMD in the indicated Vps41 backgrounds were visualized by fluorescence microscopy. Bar, 10 μm.