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. 2009 Apr 1;20(7):2041–2048. doi: 10.1091/mbc.E08-07-0699

Figure 1.

Figure 1.

Inhibition of PI3K activates FKHRL1 and represses Survivin in human NB cells. (A) SH-EP cells expressing an ECFP-FKHRL1 fusion protein were treated with 40 μM Ly294002 for up to 40 min and analyzed by live cell fluorescence microscopy in an Axiovert200M fluorescence microscope. (B) Cytosolic (cyto) and nuclear (nucl) extracts were prepared from SH-EP cells treated with 40 μM Ly294002 for 1 h. Endogenous FKHRL1 expression was determined by immunoblot. Lamin A/C (nuclear) and α-tubulin (cytosolic) served as controls for the purity of subcellular fractions. SH-EP cells were treated with 0, 10, 20, and 40 μM Ly294002 for 45 min, and immunoblot analyses of phospho-PKB-Ser473 and GAPDH as loading control were performed. (C) SH-EP and STA-NB15 cells were cultured in presence of 40 μM Ly294002 for 2 h. Survivin expression was determined by immunoblot. GAPDH was used as loading control.