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. 2009 Apr 1;20(7):2041–2048. doi: 10.1091/mbc.E08-07-0699

Figure 4.

Figure 4.

Transgenic expression of Survivin inhibits FKHRL1-induced apoptosis in SH-EP/FKHRL1 cells. (A) SH-EP/FKHRL1 cells were infected with the retroviral vector pLIB-Survivin-iresYFP and sorted by FACS. The transgenic expression of Survivin in SH-EP/FKHRL1-Surv cells was analyzed by immunoblot. α-Tubulin was used as loading control. (B) SH-EP/FKHRL1-Ctr and SH-EP/FKHRL1-Surv cells were subjected to apoptosis detection by PI-FACS analysis after addition of 75 nM 4OHT for 48 and 72 h. Each panel represents the mean ± SEM of three independent experiments.