Figure 2.

dap mutants have increased Cyclin E/Cdk2 activity. (A) Wild-type germarium, labeled with α-Cyclin E (A; A′′′, red) and α-α-Spe antibodies (A′; A′′′, green) and DAPI (A″; A′′′, blue). Cyclin E is expressed in the mitotically active cysts in region 1 and in 16-cell cysts in early region 2a (number of cells in the cyst is determined by the fusome branching pattern, arrowhead), is absent from late region 2a and region 2b, and reappears in region 3 as the nurse cells enter the endocycle (arrow). (B) Wild-type and (C) dap^−/− mutant clones stained with α-MPM2 (B, C; B′, C′, red), α-C(3)G (B′, green), and α-GFP (C′, green) antibodies. dap^−/− clones are identified by the absence of α-GFP staining. Note that MPM2 and C(3)G are never expressed in the same cysts. (C) In region 2b of dap^−/− ovarian cysts, all cells have α-MPM2 positive histone loci bodies (arrowhead).