Figure 3.

γ-H2Av foci accumulate in dap^−/− ovarian cysts and are independent of meiotic DSBs. Wild-type (A), dap^−/− mutant clones (B), mei-P22^P22/P22 (C), and dap^−/−; mei-P22^−/− double mutant germaria (D), stained with α-γ-H2Av (A, B, C, D; B′, red) and α-GFP (B′, green) antibodies. dap^−/− clones are identified by the absence of α-GFP staining. (A) In wild-type, γ-H2Av foci are restricted to region 2a of the germarium, where meiotic DSBs form, whereas in B, dap^−/− mutant cysts they increase in number (B, arrowhead) and persist into region 2b (B, asterisk). (D) The DNA damage induced in dap^−/− cysts is not suppressed in a mei-P22^−/− mutant background.