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Stimulation of Flag-NS2-NS3-GST cleavage by myc-NS3(41–213) in trans. (Upper) Increasing molar amounts of plasmid pmyc-NS3(41–213) are cotransfected with pFlag-NS2-NS3(1–40)-GST. (Lower) The resulting increase in protein expression is shown. The metabolically labeled cleavage product Flag-NS2 is enriched by denaturing RIP and detected by autoradiography (see also Fig. 2).
