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Sensitivity of FGFR1 and Csk to redox regulation. (A) WT FGFR1 catalytic domain and a Cys-488–Ala mutant were purified. (B) WT Csk and a Glu-205–Ala mutant were purified. (C) The kinase activity (turnover number) of FGFR1 and FGFR1–Cys-488–Ala as a function of DTT concentration in the kinase assay. (D) The kinase activity (turnover number) of Csk and Csk–Glu-205–Ala as a function of DTT concentration in the kinase assay.
