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. 2009 Mar 16;106(13):5159–5164. doi: 10.1073/pnas.0806671106

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Fig. 1. — Chk1^+/− mice display multiple mitotic defects and increased binucleation. (A and B) Western blot analysis of whole mammary-gland extract from Chk1^+/− (n = 4) mice shows 48% of Chk1(anti-Chk1(G4)) expression relative to Chk1^+/+ mice. β-actin was used as loading control. (C) Merged images of Chk1^+/+ pMECs undergoing mitosis and cytokinesis or Chk1^+/− pMECs undergoing various mitotic defects were stained for Aurora B (green), alpha-tubulin (red), and DNA (DAPI blue). (D) Bar graph illustrates that 18% of Chk1^+/− pMECs (n = 88) displayed multiple mitotic defects and 55% of Chk1^+/− pMECs were binucleated as compared with Chk1^+/+ pMECs. ∗, P = 0.001; ∗∗, P = 0.001; and ∗∗∗, P = 0.03. All images have 15 micron scale bars at 63× magnification.