Fig. 2.

Kinetics of LPS-induced miRNAs in PMN and monocytes. PMN and monocytes were cultured for the indicated times in medium alone (- -▵- - PMN, - -○- - monocytes) or in the presence of 100 ng/ml LPS (—▴— PMN, —●— monocytes). miRNA fraction was purified and miR-9, miR-155, miR-132, miR-146a, miR-146b, miR-187, miR-125a, miR-99b, and let-7e expression was determined by RT-qPCR and normalized to the let-7a levels, as described in Materials and Methods. The results are expressed as fold change and are representative of 3 individual experiments.