Fig. 2.
tipDCs fail to accumulate in the lungs of CCR2−/− animals. (A) Representative FACS plots of total lung homogenate from either CCR2+/+ (B6) or congenic CCR2−/− animals stained with mAbs to Ly6c and CD11b on day 5 after infection with 102 pfu of PR8 (sublethal infection). (B) Host morbidity presented as mean of percentage original body weight ± SEM after infection with 103 pfu of PR8 (lethal infection). Regression analysis did not reveal a significant difference in weight loss between the two strains of mice (strain effect, P = 0.22; interaction effect, P = 0.07). Results are representative of two independent experiments (n ≥ 5). (C) Elimination of tipDC recruitment to the lungs does not protect against lethal influenza challenge. Results of the Mantel–Cox test showed no statistically significant difference between groups (P = 0.66). Results are representative of 3 independent experiments (n ≥ 5).