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Expression of algal CmarsM7 confers arsenite resistance in E. coli. Cultures of E. coli ΔarsRBC bearing the indicated plasmids were assayed with the indicated concentrations of sodium arsenite. ▼, vector plasmid pET28a+, no arsenite; ○, pET28a-alg-arsM7, no arsenite; □, vector plasmid pET28a+, 50 μM As(III); ■, pET28a-alg-arsM7, 50 μM As(III); ▲, vector plasmid pET28a+, 100 μM As(III); ◇, pET28a-alg-arsM7, 100 μM As(III). The data represent the average of 3 replicate cultures.
